So... being the life-long geek I am, I decided to take a look at a batch of ReefChili i had just reconstituted prior to feeding the tank. Put a drop on a microscope slide and was astounded to see dozens of motile, paramecium-like protozoa skittering around the field-of-view. My first thought was, 'nah... these had to have come from the tank water'. So I took a sample from my GAC/GFO outflow where I take the water and checked that. Nothing. I mixed another small batch of ReefChili and there they were! Dozens of very alive protozoa (in addition to all sorts of other stuff that was also clearly viable and intact). I was amazed. Geek report over...