Dawn,
When removing the sand from the old system use a flat kitchen utensil & take the very top level of the sand & use that. That is the aerobic level & very good for seeding the southdown with minimal ammonia spike. I didn't do that when I changed my system over & had an ammonia spike because I mixed the sand from the deep areas (anerobic) with the aerobic & then mixed it with the southdown. very bad!!!! When my tank sprung a leak I did it the way I mentioned at the top of this page. This was done in early spring with the replacement tank. I had no spike at all & lost nothing in the changeover. (other than 70% of my sps due to them being in a tub too long without a skimmer hooked up) They perished in the tub & not inside the new tank. They never made it that far but what did survive in sps were beat up pretty bad & they recovered fine in the new system that was just set up.