Thks, I wanted to be sure it was coming out clearly with the differing posts in this thread.
What's the pocillipora specific substance? Pocilliporian or something like that?
The bright pastel colors you see in some peoples tanks are usually lack of zooxanthellae due to extremely low nutrient levels, if any at all, which can be accomplished with the help of vodka/sugar dosing. My skimmer was definitely accumulating more skimmate when I was dosing. There are MANY questions and a whole lot of REAL answers.
Not true in the least lack of zooxanthellae doesnt cause bright colors...... It causes bleaching the colors are the zoo. Also they do not need high nutrient levels infact they thrive without nutrients. No offense but when you give REAL answers you may want to use real facts to back them up 6 years in marine biology classes back up my statement about coloration being the symbiotic algea. when they loose there zoo. They bleach plain and simple. Vodka dosing is a aproach and if not done perfectly is risky Period. If you look and seriously look you will find that everyone who has dosed in majority no longer do and for a reason its not efective in the long run and macro refugia is a better solution. What happens when salinity reaches 1.030 with a calcium concentration of 500 and u add 1ppm of alcohol? SNOW I could go on for days... I use macro refugia and high volume skimmers my corals and almost all of them grow at a rate of at least an inch a month if not more and I have gone months without a water change or addition of elements of anykind without any form of parameter fluctuation. I have an extreme over population of fish high waste and o nitrates, 0 nitrites, 0 phosphates alk of 12 calcium of 449ppm, magnesium is pretty high. I could go all day but I have to go get some lunch
You're corals were most likely turning brown because of phosphate, which is far more important to keep in check than nitrate is imo. GFO specifically targets phosphate iirc.
The carotenoids are produced to protect the coral against uv radiation.
it is generally a mistake to ascribe fluorescence to chlorophyll
and a definite error to link it to peridinin, beta-carotene and some other photopigments
by extension, the idea was formed within the hobby that ultraviolet
energy is necessary for colorful corals (we know now that these sunscreens
– MAAs for mycosporine-like amino acids – are colorless and do not lend color.
mycosporine-like amino acids are responsible for UV protection. They are colorless.
They block UV. They need to both block UV and allow the zooanthellae access to light for photosynthesis. The coloration in corals is not well understood at this point but a great deal of research is being done. Florescent and colorful proteins are extremely important as biomarkers. This article goes through some details.
http://www.advancedaquarist.com/2006/9/aafeature
Not to derail the thread, but I didn't feel this question deserved its own thread...
GFO targets Phosphate, removes it, correct?
So would gfo(not sure what that is, honestly) work better than a phosphate reactor with phos-zorb?
Green fluorescent proteins (GFPs) and GFP-like proteins are those fluorescent pigments not intimately associated with photosynthesis (coral fluorescence is not due to the color of zooxanthellae!).
Universally, colored carotenoids provide photooxidative
protection against the effects of singlet oxygen
and radicals generated in the presence of light and endogenous
photosensitizers such as chlorophylls, heme, and
protoporphyrin IX (13). During photosynthesis carotenoids
can transfer absorbed radiant energy to chlorophyll molecules
in a light-harvesting function, dissipate excess energy
via the xanthophyll cycle in higher plants and certain algae,
and quench excited-state chlorophylls directly (14, 15).
GFO = granulated ferrous oxide. It's pretty expensive stuff unless you can find an online dealer that sells wholesale in bulk. IIRC, phos-zorb is alluminum oxide and is not advised for use in a reef tank by a lot of reefers, even though it says invert safe on the container. Also, phos-zorb may leach phosphates back into the system unlike gfo. I don't think it last nearly as long as gfo either.
This thread was derailed along time ago. Sorry Leroy.
"http://reefkeeping.com/issues/2008-08/nftt/index.php"
This article is like an infomercial. You know the ones where they describe
the scientific weight loss system and show picture of people that lost weight.
They then use a list of "Common Questions" like how will this increase my metabolism?
The only difference here is they show good looking corals or tanks instead of people who lost a 100 lbs.
I have seen several tanks that the corals have turned brown or faded in color because of dosing including my own. Why don't they show those pictures also?
They do mention the color loss and refer you to "amino acid addition"
Do I get 1 free bottle of "amino acids" with my dosing kit if I buy now?
There is more than enough carbon in your tank. If you want more feed your
fish more. Your corals are probably adding more sugars than dosing at those
levels. Just because you read it on the internet doesn't make it true.
Most of the claims of the vodka dosing articles and threads are
just made up reasons to justify it. Just like an infomercial.
Ozone will kill bacteria. (no exceptions) It is better than bleach.
If you allow it into the tank it will irritate your corals.
I wonder if some of the "affects" of vodka dosing are from irritating corals.
This causes them to create mucus and could be a source of the string like
structures. If corals are expending energy producing more mucus, they are
not using it to grow. The loss of color or browning of corals caused by dosing
in some cases also indicates the zooxanthellae are involved in this process.
Many questions no real answers
Telling people something is science when it is not is an insult to scientists. They spend the
time to do the proper research. I understand that people get emotionally attached to their particular formula for success. I have seen many of these fads over the years.
People had success with trickle filters,under gravel filter and not using protein skimmers.
In many cases, if you have a protein skimmer, good lighting and good current you should
succeed whether you add vodka,amino acids, sugar or whatever.
There is no science in this article and many of the threads. I took the time to read through 60 pages of one of the major threads and I read the article.
No there is not any real answers. There are no controlled experiments, No data with any statistical significance, no theory that explains it well and no agreement on what you would be measuring if you were to create a study. There is a whole lot of speculation
with made up answers. That is why a compare it to an infomercial.
Here are some examples from the article
They are not even sure what it does. For example this quote.
Now that you have bought into questionable bacterial biomass statement. You are
expected to believe this
There are no new macromolecules Any creation of bacteria that "take up"
NO3 and PO4 do not require these magic new macromolecules
Let me translate this. Since some people have had success it must be true. Look at the nice tanks they have on reef central.
heterotrophic bacteria has no meaning in the context of this statement.
My first question would be if heterotrophic bacteria
are not carbon limited in an aquarium, how does adding a carbon source help?
There are many other unsubstantiated statements in this article but it would take all day to go through them all.
Nathan is a undergraduate student at OSU as far as I can tell.
His advisor is Dr Gustavo W. Leone
Dr Gustavo W. Leone
808 Biomedical Research Tower
460 W 12th Ave
Columbus, OH 43210
2008 - present Advisor. Nathaniel Walton, Methylation of DNA Inhibited Indirectly Using Interference RNA, The Ohio State University , Columbus, OH. Undergraduate.
There is no absolutely and I understand that you don't like your beliefs questioned.
How do you improve a hobby when no one questions the latest snake oil salesman?
One other note This is the Advanced Reef Topics and I hope would require a little more science
Isn’t an infomercial where someone is trying to sell a product
In fact the article was a summation of people’s experiences over the past few years.
The science is not present as much of it has yet to be demonstrated in seawater. Also, there are potentially many processes going on that have yet to be elucidated. By placing science within the article, I would be slapping the face of my profession and disgracing RK. However, if you read through the threads listed I do believe scientific articles have been posted that clearly demonstrate the strong possibility that bacteria do what the article states.
I am confused about this replyIn this comment “ozone will not kill bacteria, no exceptions.” That’s like saying heat will cook humans, no exceptions. Ozone concentration plays a role in destruction and the ozone amounts we run and how they are usually used should not destroy bacteria considerably.
Jörg Kokott, a key contributor to the original thread, recommended the use of ozone during the duration of vodka dosing to maintain high levels of dissolved O2 in the system. This decrease in dissolved O2 is indirectly observed in ORP meter readings after vodka addition. Though not an absolute requirement, as told from many RC participants, ozone may add an extra level of protection by increasing O2 levels during an overdose.
Gelbstoff, German for yellow matter, is reported to buildup in aquariums over time. Additions of vodka or other carbon sources have the potential to accelerate the yellowish water buildup from organics. To solve this problem, people have utilized ozonators to breakdown of the organic molecules responsible for resulting in yellowish water. Ozone is an attractive choice as it would not only breakdown the gelbstoff but will also add O2 to the water in case of an overdose. For people not interested in running ozone other solutions for this problem are the addition of granulated activated carbon or through regular water changes.
Is it Ethanol through an alcohol dehydrogenase pathway into the citric acid cycle (Krebs) will improved bacteria growth in your aquarium? The bacteria uptake the nitrogen and phosphates. They are removed by protein skimming and thus reduce nitrogen and phosphates. “that carbon is not a limiting element in reef tanks.” The food you add to an aquarium is a substantial source of complex and small molecules of carbon compounds. How do you see your dosage of ethanol in comparison to these food additions? How do these dosing levels compare with the simple molecules provided by coral mucus in an aquarium.
Why not add sugar or sodium citrate?
I am confused about this reply
The comment was Ozone will kill bacteria. (no exceptions) It is better than bleach. In the article ozone was described as having two benefits
1. Provide additional oxygen for the aquarium in of overdose
2. Break up organic molecules causing yellow color in the tank.
If you have good protein skimming your oxygen levels should reach saturation.
Add enough ozone to change this should be more than enough to have a major affect on bacteria and other organisms in your tank.
If it is enough to breakup organic molecules causing yellow color it should be enough to kill bacteria. Without rates of ozone addition and flow, this is a
difficult question.
I believe this paper is an excellent article that would answer many questions.
IInorganic nutrient limitation of oceanic bacterioplankton
DOC in the range of 60-80 uM (microMolar)
Although most (-70%) of this DOC is thought to be old
(~4,100 yr; Bauer et al. 1992) and biologically refractory,
between 6 and 19% (Sondergaard and Middelboe 1995;
Carlson and Ducklow 1996) of the total may be biologically
labile (LDOC) and turns over on time scales of hours to
days. If we assume that the DOC distribution and the
LDOC : DOC ratios at our study sites were similar to other
oligotrophic regions, LDOC would be -4-15 PM C.
In contrast, DOC rather than PO,
seemed to stimulate the bacterial activity in the Sargasso Sea
near Bermuda during January (Carlson and Ducklow 1996);
thus, the type and magnitude of nutrient limitation may show
seasonal and spatial differences.
I have realized the wisdom of this acronym many times in my relatively short life.
KEEP IT SIMPLE STUPID = KISS
I am by no means implying any stupidity. I am in the same boat with my 180 mixed reef as those in the thread find themselves. I am sharing my thoughts here to attempt to help those appreciate my revelation and success with something simple.
HUSBANDRY
I increased the quality of my source water RO/DI improvements... namely better cartridges, more resins, looking into catalyzed carbon now...
This improvement was cheap compared to GFO by the 5 gal bucket. Cartridge life is long in comparison to GFO's efficiency over time.
Increase volume of water changes / algae removal / refugium and total system water volume was increased by 40%. The skimmer is cleaned every three days or so along with sand bed vacuums weekly with water changes.
I also increased water movement in the display with two 5 gallon Borneman Surge Devices and a couple of Koralias along with my original Rio 3100 and double 600 gph returns. Orig. system water movement was 1800 gph and is now around 4200 gph (depending on surge waves). I like the Borneman buckets and they don't put out microbubbles, just some larger ones in the beginning and end of a surge.
So, in short... I almost used GFO until I rinsed it to put it in a filter bag and it looked like the Rio Grande during flood season. I used seachem phosguard and that made my corals (leathers and zoos unhappy and grow slow) I removed both and used Carbon only.
Natural seems best and is cheaper, in my opinion. When I get a chance, I will post pics on the system and it's parts. I am a reefer on a budget, a tight budget expecting a little one. Everything I do must pass a max. efficiency test. So, I hope my words help with your decisions. I am not saying Vodka is bad, it livens up some nice drinks at my bar next to the tank, but I am skeptical as to whether my Paracanthurus wants to indulge in a daily shot. I say step up the basics of aquarium keeping first to get to where you want to be. If that is not enough, start a Vodka experiment.
I say all of this with the utmost respect, and want those to know that I am not just a hack, I am an ecologist and have professional experience with some of the same issues, on a much larger scale.